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In the first, some variants are fixed by positive selection to compensate deleterious mutations in other epistatically interacting positions.
From the CD11b column we isolated microglia by positive selection and astrocytes by depleting microglia.
Stable dual‐gene expression was confirmed in both T‐cell types, permitting enrichment by positive selection with Rituxan.
B cells were purified from splenocytes by positive selection with CD19 MicroBeads (Miltenyi Biotec) in accordance with the manufacturer's instructions.
These patterns are caused primarily by positive selection, so our scan missed other types of selection.
Therefore, increased susceptibility to disease may accompany a fitness-increasing mutation introduced by positive selection.
In many proteins, the amino acid substitutions caused by positive selection are non-random [32], [33].
CD14+ monocytes were isolated from PBMCs by positive selection with CD14 microbeads (Miltenyi Biotec).
We first isolated CD34+ cells from donated human bone marrow samples by positive selection (Fig. 3A).
CD4+CD25+ T cells were purified by positive selection using anti-CD25 mAb-coated microbeads.
Rapid evolution can mainly be explained by relaxation of purifying selection or by positive selection.
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