Sentence examples for by positive isolation using from inspiring English sources
CD4+ and CD8+ T cell subsets were purified by positive isolation using microbeads (Miltenyi Biotec).
PDCs were purified by positive isolation using anti-BDCA-4-conjugated magnetic microbeads, and BDCA-1 mDCs were purified using anti-CD1c-conjugated microbeads (both Miltenyi Biotec, Bergisch-Gladbach, Germany) after B cell depletion.
T-cells were isolated from pathologically healthy donor buffy coats (National Blood Service) using sequential negative cell selection with magnetic beads coated with anti-CD3, followed by positive cell isolation using magnetic bead coated with anti-CD8 (Miltenyi Biotec Ltd ,Surrey, UK).
In some experiments, CD8+ T cells were positively isolated using a CD8 Positive Isolation Kit (Dynal, Oslo, Norway).
VHSV-qRT-PCR positive samples were processed for virus isolation using EPC (epithelioma papulosum cyprini) cells [40].
Tregs were isolated by positive selection using Mouse Treg Isolation kit from Miltenyi Biotec (Auburn, CA), as per the manufacturer's protocol [5].
Purification of CD34+ cells was by positive selection using MACS Direct CD34 Progenitor Cell Isolation Kit (Miltenyi Biotech, Bergish-Gladbach, Germany).
Ovalbumin (OVA -specific CD8+ T cells were isOVA -specifiche spleen of OT-I transgeniCD8+ce by posiTive selecells using magnetic cell separation (MACS, mouse naïve CD8+ T-cell Isolation Kit, Miltenyi Biotec).
Isolation of CD14+ cells was performed with magnetic beads (CD14 Microbeads, Miltenyi Biotec, Madrid, Spain) by positive immunoselection using the autoMACS Separator (Miltenyi).
For isolation of CD4+CD25+ cells, CD4+ lymphocytes were incubated with anti-CD25 microbeads for 15 minutes at 4°C, followed by positive selection using an additional separation column.
