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The second, referred to as 'positive sera' from this point forward, was created by pooling sera from a different cohort of 24 JJAV allergic individuals with quantifiable venom-specific IgE.
Two subgroups were formed from the DHT hamsters depending on whether the individual ejaculated after replacement treatment (4 hamsters) or failed to ejaculate (6 hamsters generated 4 samples derived by pooling sera from 2 pairs of hamsters with insufficient blood volumes for individual assays).
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KETc1 was then selected after being recognized by pooled sera from cysticercotic pigs during a second screening [28].
The negative control sera were from unimmunised mice and the positive control sera were pooled sera from previously immunised mice that had been tested by ELISA and found to have antibodies that bound TNF.
Using ion-exchange chromatography, pooled sera from normal individuals and cancer patients were fractionated by differential salt elution.
Because serum containing high or moderate levels of sFas might also differ in the concentrations of the cytokines mentioned above, we pooled sera from four sepsis patients before immunoprecipitation of sFas by anti-Fas antibodies (ZB4).
Pooled sera from eight egg-allergic patients were used to probe the membrane.
Pooled sera from M. tuberculosis culture-positive patients were used as positive controls (OD, >1.0).
We pooled sera from five patients with different disease presentations in order to detect a spectrum of reactive antigens.
No bands were identified using pooled sera from healthy controls.
Diluted 1 100 pooled sera from vaccinated mice were added to the cells and cell proliferation was determined by MTS assay.
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