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MDR works by pooling genotypes from multiple loci to create new variables before subsequent associative analysis.
At the heart of the MDR approach is a feature or attribute construction algorithm that creates a new variable or attribute by pooling genotypes from multiple SNPs (Moore and White, 2006).
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We then pooled genotype data from the studies.
We pooled genotype data from studies within CIMBA that had previously genotyped polymorphisms rs1042522 and rs2279744 (see Table 1).
Each cDNA library was tagged with Multiplex Identifiers (MID) barcode adaptors, and two independent multiplexes were created by pooling one sample from each genotype and treatment.
To this aim, RNA samples were prepared by pooling RNAs from 5 brains of 6-week old mice of each genotype.
Further, we have analyzed the data by pooling the ID genotype with II and DD genotypes respectively.
Therefore by pooling a smaller number of amplicons, we could have genotyped more individuals.
Validation was performed on an independent set of biological replicas by analyzing pooled samples from four additional mice per genotype.
The effect estimates (ORs) for GCLM- 588 TT were strongly deviant from the CT genotype, which prevented us from pooling these two genotypes.
Pooled data from genotypes in Table 1.
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