Sentence examples similar to by pooling equal volume from inspiring English sources

The samples were prepared by pooling equal-volume aliquots from groups of ten women.

The artificial template described in the Results and Discussion was generated by separately amplifying the repeat-containing regions from Vigna and Cucurbita templates with PCR, gel-extracting the products with a QIAquick Gel Extraction Kit (Qiagen Inc ., then pooling equal volumes of the two PCR products into a single mixture (Fig. S2).

We therefore pooled equal volumes of stationary-phase cultures of each functional mutant and recovered the pooled genes with a mini-prep.

A common reference was created by pooling equal amounts of total RNA from all tissues.

An internal control was created by pooling equal amounts of protein from all of the samples.

Serum samples were collected, pooled by equal volume and subsequently subjected to the neutralization assay two weeks after the second immunization.

The samples of each study group were divided into two subsets (n = 5 each) and, when needed, samples were pooled by equal volume.

Note that amplification products generated using the non-γ-c and the γ-c OR clade primers were pooled using equal volume aliquots before the ligation reaction.

PCR products generated by four different fluorescence dye-labeled primers were pooled in equal volumes and 1.5 μl each of FAM- VIC- NED- and PET-labeled product were mixed with 7 μl of formamide (Applied Biosystems), 0.25 μl of the GeneScan™ 500 LIZ® Size Standard Applied Biosystemss) and 2.75 μl of distilled water.

The indexed cDNA libraries were diluted to an average concentration of 10 nM and pooled in equal volumes (10 μL of each library) to generate the final mixed cDNA pool for sequencing.

Libraries were diluted to equal molarity and finally pooled by using equal volumes of each library.