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Reference RNA was obtained by pooling equal amount of total RNA extracted from male and female wildtype fish.
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Bulked segregant analysis (BSA) by pooling equal amounts of DNA from 20 short-root or 20 normal-root plants was used to determine the chromosome location of the mutant gene.
The common reference was generated by pooling equal amounts of targets from the adult gonad, brain, kidney and rest-of-body of both sexes.
A common reference was created by pooling equal amounts of total RNA from all tissues.
An internal control was created by pooling equal amounts of protein from all of the samples.
Each cDNA library was constructed by pooling equal amounts of total RNA from all molt cycle stages.
Each cDNA library was constructed by pooling equal amounts of total RNA from all moult cycle stages.
Each pool of the two pools/group was prepared by pooling equal amounts of the same three or eight RNA samples [Additional file 10].
A time-matched vehicle control pool was made for each chemical by pooling equal amounts of RNA from each of the control animals.
This library was constructed by pooling equal amounts (333 ng) of cDNA from DiG01, DiG02, GDG01, Hae01, Hae02, Hae03, Hae04, Gll01, MxT04 libraries.
The 454 reads have been obtained from a normalized library constructed by pooling equal amounts of mesocarp RNA from stages S1, S2, S3 and S4 (GenXPro GmbH, Germany).
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