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Normal pooled plasma was collected at the Maastricht University Medical Center (Maastricht, The Netherlands) by pooling PPPs from 80 healthy volunteers.
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One milliliter of each control (pooled PPP and 0.5% DMSO diluted in pooled PPP) was added to a tube containing a WB clot (method described as above), which was then covered by parafilm.
Human pooled PPP was prepared and was strictly processed by collecting 9.00 mL of WB into two trisodium citrate tubes (4.5 mL each) from participants with O blood group (n = 20).
In order to better understand the relationship of "bacterial-like" PPP phosphatases in eukaryotes to each other and to bacterial PPPs, we attempted to extend our previous phylogenetic analysis of eukaryotic PPP phosphatases [ 25] by including PPP sequences from a number of bacteria and archaea.
The PPP from all the participants was pooled and sample integrity was tested using coagulation parameters.
First, we demonstrate improved methanol assimilation in the presence of small amounts of yeast extract by expressing the non-oxidative pentose phosphate pathway (PPP) from Bacillus methanolicus.
The row pools from each plate were pooled by hand to produce 252 plate pools.
(A and C) Micrographs of PPP with added thrombin to form extensive fibrin fibres from 2 individuals with type 2 diabetes; (B and D) PPP from same individuals, but exposed to 2 ng.LPS-bindingding protein followed by addition of thrombin.
He took against the PPP from the start.
To generate PPP tolerant derivatives, Line2 and Line3 cells were exposed to increasing doses of PPP from 10 to 500 nM over an 80-week period resulting in derivatives with different levels of PPP tolerance, as previously reported [7].
The decision is based on the features we extracted from the genomic context, the prediction itself and the other two closest predictions in the surrounding region, either by the current PPP or by other PPPs.
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