These MP reactor systems are characterized by polychromatic output.
Ex-vivo intra-hepatic lymphocytes from HCV mono-infected and HCV/HIV co-infected individuals were assessed for immune responses to HIV and HCV antigens by polychromatic flow cytometry.
A fraction of cells was removed and analyzed by polychromatic flow cytometry.
The data analysis program, Simplified Presentation of Incredibly Complex Evaluations (SPICE, version 4.1.5, Mario Roederer, Vaccine Research Center, NIAID, NIH) was used to analyze and generate graphical representations of T cell responses detected by polychromatic flow cytometry.
To assess the total magnitude, functionality, and phenotypes of Ad5-specific CD8+ T-cells, we stimulated human PBMCs with Ad5 vector and measured CD8+ T-cell responses by polychromatic flow cytometry (Fig. 1A).
We compared mutagenic spectra induced by polychromatic near-ultraviolet radiation (near-UV; 300-400 nm) with superoxide anion (O2-) -dependent mutagenesis using a set of Escherichia coli tester strains.
Activated T cells were identified by polychromatic flow cytometry as CD4+CD44+CD62L- or CD8+CD44+CD62L- cells after gating on live, CD3-expressing cells.
Characterization of T cell immune activation and memory was performed in batch by polychromatic flow cytometry on frozen/thawed heparin PBMCs.
Surface expression of the differentiation marker CD57 and three inhibitory markers (PD-1, CD244 and LAG-3), previously shown to be upregulated during chronic viral infections [ 21, 24, 25], was determined by polychromatic flow cytometry.
Furthermore, a detailed phenotypic analysis of blood immune cells (B cells, dendritic cells, myeloid-derived suppressor cells, macrophages, NK cells and T cells) will be performed by polychromatic flow cytometry.
