Table 1 Thickness of AZO films simulated by SE and measured by SEM, respectively Methods 150 cycles 300 cycles 450 cycles SE (nm) 26.0 50.3 75.6 SEM (nm) 24.8 47.3 75.4 Fig. 1 Refractive index (n) and extinction coefficient (k) were simulated by point-by-point analysis using the data from SE measurements.
A group of markers for E26C13 was defined by two-point analysis using a LOD threshold of 8.
A group of GGA5 markers was defined by two-point analysis using a LOD threshold of 6.
Imbalanced chromosome segments (ICS) were identified by change-point analysis using a circular binary segmentation algorithm [ 29].
A group of markers for GGA16 was defined by two-point analysis using a LOD threshold of 8.
Linkage distance and marker order were calculated by two-point analysis using mapmaker/exp 3.0b for Unix (http://www.nslij-genetics.org/soft/mapmaker), the Kosambi mapping function, a maximum intermarker distance of 36 cM, and a minimum LOD score of 4.0.
The MCID was determined by an optimal cut-point analysis using both the raw (MCID) and percentage (MCID%) change scores.
After PCR amplification, the alleles were detected by means of end-point analysis using SDS and TaqMan Genotyper software (Applied Biosystems, Foster City, CA).
Furthermore, a point-by-point analysis [20] was used to calculate the n and k at the whole wavelength, and the results are presented in Fig. 1.
The specificity of all reactions was determined by melting point curve analysis using one cycle at 95°C for 5 seconds and 65°C for 1 minute followed by a heating up step to 97°C in a continuous way of fluorescence acquisition.
Mechanical testing of the cortical bone was assessed by 3-point bending analysis using a Zwick materials testing machine (Zwick A.G., Ulm, Germany) with a 50 N loading cell, where the span was set at 5.5 mm and the cross-head speed was set at 1 mm/min.
