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However, the differences in ACT9 expression in the different tissues did not explain the tissue-specific expression of ALS3 and MATE, as was shown by plotting transcript levels relative to ACT9 (data not shown).
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Plotting transcript fold changes levels shows a high correlation among the biologically replicated sequencing runs as indicated by Euclidean distances.
Our aim was to assess, in samples taken from all six plots, transcript-level expression of fungal genes encoding lignocellulolytic and chitinolytic enzymes.
The mean of three different experiments was plotted and transcript level expressed as arbitrary units.
We normalized each transcript level by multiplying raw transcript levels by the scale factor (as in [6], [11]).
Transfection efficiency was found to be ~75% (Fig. 8J, p < 0.001 against TGFβ + ITF2357) as determined by Id3 transcript levels.
CTB8 transcriptional activator controls cercosporin production by controlling gene transcript levels.
desi3a-1 was confirmed by comparing its transcript levels with that of Col-0 (Supplementary Fig. 14).
By contrast, antisense transcript levels significantly dropped during invasion.
This was confirmed by plotting calpain activity vs calpastatin levels.
Fold change in transcript levels was plotted using Prism 5 software (GraphPad, La Jolla, CA).
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