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The concentration of ammonia was calculated from straight line equation generated by plotting the standard graph.
We assessed the potential publication bias by visual inspection of the funnel plot produced by plotting the standard error against the log OR of studies.
The raw intensity cut off value of 80 was determined based on the base over proportional (C = a/b) calculation, which is generated by plotting the standard deviation of normalized values against the control values.
There was no evidence of potential publication bias as assessed by visual inspection of the funnel plot produced by plotting the standard error against the log OR of studies.
To obtain better insight into tumor heterogeneity, we quantified overall variance within each class by plotting the standard deviation vs. mean expression of each miRNA for nodular and infiltrative tumors.
The PCR condition of each MSP primer set was validated by plotting the standard curve according to various mixtures of band intensity from the MSP products with universal methylated and unmethylated control DNA [ 11, 18].
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Internal standards are required for reliable quantification in electrophoresis, so calibrations were performed by plotting the analyte/internal standard peak area ratio versus the analyte/standard concentration ratio.
The linearity of each calibration curve was determined by plotting the analyte/internal standard peak area ratio (Y) against the nominal concentration of analytes (X).
The testosterone, FSH, and LH concentrations of the untreated and treated serum were run concurrently with the standards and calculated from the standard curve, obtained by plotting the concentration of the standards versus absorbance.
The linearity of the calibration curve was evaluated by plotting the absorbance of standard solutions of Cr VI) against the concentration.
Outliers were visualized by plotting the mean versus standard deviation for each comparison.
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