The calibration graph was obtained by plotting the fluorescence intensities versus the corresponding concentrations and the regression equation was computed.
At the same time, external standards of H2O2 mixed with 50 μg/mL HRP@HSNs were used to develop a calibration curve by plotting the fluorescence intensity versus the concentration of H2O2.
Kinetic assessment of ECCNSs (Figure 10b, c, d) uptake and void etoposide (Figure 10f, g, h) in SGC-7901 cell was conducted by plotting the fluorescence peak of each sample against the different incubation times of 1 h (b, f), 2 h (c, g), and 4 h (d, h).
The fluorescence intensity of the RITC-HRP@HSNs was measured by suspending the nanoparticles in 1 M NaOH, and the amount of encapsulated RITC-HRP was determined according to a calibration curve established by plotting the fluorescence intensity versus the concentration of native RITC-HRP under the same conditions (Additional file 1: Figure S2).
To determine the HRP loading capacity, RITC-HRP@HSNs were dissolved in 1 mL of NaOH (1 M) for 1 h, and the amount of the entrapped RITC-HRP was calculated from a calibration curve established by plotting the fluorescence intensity versus the concentration of RITC-HRP.
Kinetic assessment of void etoposide (Fig. 5 d2, d3, d4)) and ECCNBs (Fig. 5 e2, e3, e4)) uptake was showed by plotting the fluorescence peak at different incubation time of 1 h (d2, e2), 2 h (d3, e3), and 4 h (d4, e4).
This was achieved by plotting the integrated fluorescence intensity versus the absorbance at 488 nm (Fig. 4).
The CT values were determined by plotting the observed fluorescence against the cycle number.
The threshold cycle (Ct) values were determined by plotting the observed fluorescence against the cycle number.
Results were presented by plotting the intensity of fluorescence at 450 nm which is the characteristic novel excitation maxima formed upon Th T binding.
The analysis of data from pool competition experiments with diphenyleneiodonium chloride (DPI), by plotting the mean arbitrary fluorescence of untreated and treated pools, identified the nrt1Δ/ nrt1Δ deletant as highly enriched in the population following DPI treatment.
