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A standard curve of purified flagellin was constructed using a linear curve-fit by plotting the band intensity for each standard on the y-axis against the concentration on the x-axis.
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The measurements are further confirmed by plotting the energy band diagram of the structures, calculating the quantum tunneling probabilities, and analyzing the charge transport mechanism.
A calibration curve is drawn by plotting the percentage of shifted material (bands C1+C2) as a function of the amount of HMGB1 on a semi-logarithmic scale.
A calibration curve was obtained by plotting the intensity of SERS signal of FC band at 1178 cm−1 versus the concentration of antigen.
Sensitivity was determined by plotting the LSPR wavelength of the 500 760 nm band as a function of the measured refractive index.
By plotting the Tauc graph ((sqrt {alpha hvartheta }) versus hϑ), the band gap energies of the TiO2 nanotubes can be evaluated with the extra plotting line to the x-axis from Fig. 4.
Gels were then visualized on a Typhoon (GE), and Image J software was used to quantify the area of bands on each lane by plotting intensity of each band intensity and integrating the area under the curve for each band.
Quantification of band intensity for each blot is plotted in Figure 4C and D. For AtClpD, only the lower band was plotted (the upper band behaved in a similar way).
The chemical maps at the single microsphere level were generated by measuring and plotting the intensity of specific bands for Exenatide (amide I and amide II) and PLGA (C=O bond).
For the inhibition ELISA results, the distribution of the data was examined first by plotting percent inhibition, with the data aggregated into bands of 10%.
A linear relationship between the two was established by plotting a regression line, with the upper 95% simultaneous confidence band also presented.
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