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The reciprocals of reaction rate (consumption rate, i.e., 1/r) and organic matter content (1/C) were compiled by plotting a Lineweaver Burk plot (1/r vs. 1/C), to determine the kinetic constants k m and r m for all sets of experiments as summarized in Table 5.
The results were analysed by plotting a Lineweaver Burk curve to determine Km and Vmax.
The results were analysed by plotting a Lineweaver Burk curve to determine the Km and Vmax.
The data were plotted as a Lineweaver-Burk curve also called the double reciprocal curve.
The final enzyme concentration for the assay was estimated as millimolar; Km and Vmax were evaluated by plotting the data on a Lineweaver-Burk double reciprocal graph (Prism 5 Computer Programme, Graph Pad Software, San Diego, CA, USA).
Inhibitory constant (K i ) was derived by plotting slopes obtained from Lineweaver-Burk plots versus inhibitors concentration.
A Lineweaver-Burk plot revealed that compound 4f acted as a mixed-type xanthine oxidase inhibitor.
The results from a Lineweaver-Burk plot indicated a mixed-type inhibition for compound 7l with BChE.
A Lineweaver-Burk plot and molecular modeling study showed that compound 7f targeted both the CAS and PAS of AChE.
A Lineweaver-Burk plot was generated in Microsoft Excel and kinetic parameters determined from it.
The Km values were obtained from a Lineweaver-Burk plot [ 24].
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