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First we determined the bacterial load by plating a defined number of FACS sorted cells onto blood agar plates (Figure 5C).
The SCA output is controlled by plating a defined number of cells per well (25 000) onto the ELISPOT plate for final counting; however, growth rates for individual cell lines were also estimated (Supplementary Material, Fig. S2).
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The competent cells of the lignin-tolerant strain MITXM-61L53 were treated by electroporation, and plated on a defined glucose-containing agar medium with 0.75 g L−1 4-HB.
From the culture broth after a total of 4 generations (22 days), 20 colonies were randomly isolated by plating for single clones on a defined agar medium with 16 g L−1 glycerol and tested again for growth in flasks with a defined medium containing 100 g L−1 glycerol.
The ions produced are repelled and focused by plates with defined potentials before entering the analyzer [ 21].
After a total of four generations (20 days), at least ten colonies were randomly isolated by plating for single clones on the defined agar medium and tested again for growth in flasks with the defined medium supplemented with the inhibitor at appropriate concentrations.
Unsporulated cells were removed by β-glucuronidase digestion, and a defined number of spores was plated onto rich medium.
Unsporulated cells were removed by β-glucuronidase digestion, and a defined number of spores was spread onto plates with rich medium.
The subchondral bone plates of the femoral heads were examined by scanning electron microscopy and a defined planar section was photographed.
Plating efficiency was defined as number of colonies divided by the number of plated cells.
A – defined by highest percentage.
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