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The fungus was found to be positive for extracellular α-amylase activity [ 3] by plate screening method (Supplementary Figure S1; see Supplementary Material available online at http://dx.doi.org/10.1155/2015/245649).org/10.1155/2015/245649
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The activity of polysaccharide cleavage enzymes has usually been evaluated by qualitative plate screening methods and quantitative colorimetric or chromatographic assays.
This commonly used on-plate screening method employs an engineered lacZα protein with an internal multiple cloning site (MCS).
This putrescine oxidase was discovered by using a plate-based screening method for oxidase activity, which has been used before to screen for improved mutants during directed evolution experiments (Alexeeva et al. 2002).
Several methods have been proposed to screen proteolytic (including keratinolytic) enzymes, including keratin-baiting, plate screening, spectrophotometric methods, and sequence-based amplification.
b The halftone image generated by the screening method [11].
The 84 VRE isolated from sewage sludge were subtyped by a rapid screening method for enterococci using PhP-FS microtitre plates (PhenePlate™, PhPlate Microplate Techniques AB, Stockholm, Sweden) [ 25].
Positive (pRSETB-Xi Piromyces) and negative (pRSETB) controls were also transformed into the screening strain and plated in selective media to validate the screening method.
A screening method is presented for selecting optimal configurations for plate heat exchangers.
The screening method was based on the measurement of growth in acrolein-supplemented medium, in 96-well microtiter plate cultures.
A useful screening method for the presence of PHAs is the incorporation of the dye Nile Red into colonies on agar plates and its detection by fluorescence (Spiekermann et al. 1999).
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