Sentence examples for by plasmid integration using from inspiring English sources
The inactivation of the lantibiotic modifying enzymes LicM1 and LicM2 was performed by plasmid integration using the thermosensitive shuttle vector pMAD [55].
The integration could be done by the Gauss-Hermite method as recommended by [ 31]; in fact adaptive integration using the Numerical Algorithms Group NAGG) integration subroutine D01AMF was used here.
Carry out the integration using integration by parts.
When cells containing a docking platform marked by GFP expression are challenged with a plasmid that contains a MTX-resistance marker between two attB sites, in combination with an integrase-expressing plasmid, correctly integrated products should be resistant to MTX and should fail to express GFP; we initially tried to select targeted integration using either or both of these properties.
Uncharacterized genes were inactivated by homologous suicide plasmid integration as described previously [ 47] using pK18 mob as the vector [ 48].
By contrast, plasmid integration (ends-in gene targeting) is often associated with multiple targeted integration events but illegitimate integration is extremely rare and a targeted chromosome duplication has not been reported.
All strains constructed in this study were derived by transformation of S288c and YJM789 S. cerevisiae strains with integration plasmids using standard techniques (Gietz et al. 1995).
Plasmid integration was as described earlier using the pDUAL multicopy vector (Matsuyama et al, 2004).
To identify their physiological roles, we inactivated nine regulon genes by homologous suicide plasmid integration.
In some cases, linear plasmids engage with chromosomes in an intricate evolutionary interplay, facilitating the emergence of new genome configurations by transfer and recombination or plasmid integration.
The genomic DNA was assayed for integrated plasmid using a sensitive PCR method, and the risk of mutation due to integration of pcS/2SS plasmid following intramuscular injection in mice was negligible.
