Zoospore release was promoted by placing the solutions at 4 6 °C for 20 60 min and then returning to 20 °C.
Electrospinning (ES) was performed by placing the polymer solution into a plastic syringe tipped with a 27-gauge stainless-steel needle.
The agarose solution droplets are induced to gel by placing the tubes of solution on ice, encapsulating the islets within agarose beads (Fig. 6) [40].
Anaerobic KO2 and H2O2 stock solutions were prepared by placing the KO2 and H2O2 solutions in a sealed vial, applying a vacuum, and then using a vacuum manifold to flush them three times with N2.
After washing twice, dots were visualised by placing the membranes in ECL solution and then exposed.
Staining was performed by placing the gels into fixative solution (40% ethanol, 10% acetic acid) for 2 h and then staining them with 0.175% Coomassie brilliant blue (CBB) R350 (GE Healthcare).
The sensor parameters such as conductivity, turbidity, water level and pH are measured by placing the sensor into different solutions of water.
An antigen retrieval procedure was performed by placing the sections in Citra-solution (HK086-9K; Biogenex, San Ramon, CA, USA) inside a plastic pressure cooker, which was positioned in a microwave oven (Kenmore; Sears, Chicago, IL, USA).
Warming procedures were performed by placing the Cryotop in a warming solution (TS, 1 mol/L sucrose) for 50 60 s at 37°C and moving into a dilution solution (DS, 0.5 mol/L sucrose) for 3 min at room temperature.
Measurements were made by placing the electrodes within the culture solution of interest and measured the time required to identify readily detectable frequency changes (detection time, DT).
