Sentence examples for by placing the slides from inspiring English sources

We followed the provided instructions and pretreated the sections with hydrogen peroxide for 20 min at room temperature and then performed an antigen retrieval procedure by placing the slides in a steamer (at >99 °C) for 5 min.

The sections were rehydrated by placing the slides in three changes of xylene for 10 min each, then graded ethanol from 100 to 50%%, followed by water and Tris-buffered saline.

Cells were fixed by placing the slides in ethanol.

Following a series of TBS washes, antigen retrieval was performed by placing the slides in 1% SDS in TBS at 37°C for 20 minutes.

The probes, together with the sperm, were denatured by placing the slides on a slide warmer at 75°C for 3 minutes, and then incubated overnight at 37°C in a humidified chamber.

Prior to staining, heat-induced antigen retrieval was performed by placing the slides into target retrieval solution, high pH (DakoCytomation), and steaming them in a commercial vegetable steamer at full temperature for 30 minutes (CEBPD) or by pressure cooking in TRIS/Citrate (pH 6) for 8 minutes (alpha-smooth muscle actin, ACTA2).

The prehybridization buffer was rinsed off by placing the slide in a 50-ml tube for 2 min, first in H2O, then in 70% ethanol, and then in 100% ethanol.

The mount was rehydrated in increasing dilutions of ethanol in distilled water (70%, 50 %, 30 %, 10 0%, 10 minutes each) and then stained by placing the slide in Carmine Alum stain over night.

Rehydration and washing were carried out by placing the microscopic slides in glass jars containing approximately 60 ml of the appropriate solutions.

In brief, antigen retrieval was performed by placing the unstained slides in 0.01 mM citrate buffer (PH 6.0) and treating them in a microwave oven for 10 min. Overnight incubation at 4°C was performed for anti -pan-Trk, -p75NTR, -pc-Jun and -pJNK primary antibodies and 1 hour at room temperature incubation was performed for anti-TrkA, -TrkB and -TrkC primary antibodies.

Time-lapse imaging was performed by placing the chamber slide on the VL21 microscope surrounded by a Solent Scientific environmental chamber (Solent Scientific Limited, UK) maintaining the cells and the microscope at 37 °C.