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In this chapter, methods for characterizing GnT-IVa-deficient mice by physiological analyses to detect metabolic alterations and biochemical analyses using primary isolated pancreatic β cells are summarized and discussed.
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Physiological analyses to elucidate upstream signalling pathways revealed that LOV1 regulates the cold response in plants.
Deleted genes could subsequently contribute to the identification of the molecular mechanisms underlying the observed phenotypes by means of a candidate gene approach, validated by physiological analyses or genetic transformation [ 26].
In previous studies, the influence of phytohormones in Physcomitrella was examined by physiological analyses [ 10, 11] and in a recent survey the auxin distribution in Physcomitrella was characterised with the help of transgenic plants [ 12].
Finding the molecular mechanisms of the inhibitory action by detailed physiological analyses is a promising strategy to discover metabolic engineering targets that can improve strain robustness.
Key pathways identified by this approach to differ between the two poplar species were validated by targeted biochemical and physiological analyses as well as by non-targeted metabolite profiling applying Fourier Transform-Ion Cyclotron Mass Spectrometry (FT-ICR MS) [ 18- 20].
In the current work, we have investigated the factors underlying the prevention of chilling injuries in peaches by the application of GA at the initial stages of pit hardening, coupling physiological analyses of a responsive cultivar to global transcriptional profiling.
We are grateful to Randi Aune for contributions to cultivations and physiological analyses.
In this study, we constructed a comprehensive transcriptome using NGS technology in combination with physiological and biochemical analyses to investigate the process of starch accumulation mediated by endogenous hormones in Landoltia punctata.
Although two Canadian strains belonged to subgroup Ia, molecular and physiological analyses showed the divergence between Canadian and Japanese strains.
Plants can accumulate varying levels of Fe and the response of rice to Fe toxicity was recently summarized after comprehensive transcriptomic and physiological analyses (Quinet et al. [2012]).
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