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In T47-D breast cancer cells, the activation of moesin was related to the concentration of E2 and was triggered by physiological amounts of the steroid (Fig. 2C), whereas in MDA-MB-468 cells no change in moesin phosphorylation was detected throughout the range of estradiol concentrations (Fig. 2D).
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Probably the most conclusive experiments are performed by co-immunoprecipitating endogeneous, and thus physiological amounts of, proteins.
Human liver spheroids were formed combining differentiated HepaRG cells and HHSteC using 384-well spheroid microplates (Corning) in HepaRG medium containing physiological amounts of insulin (1 nM).
NB analyzed by SDS-PAGE and stained by Coomassie blue showed a similar smearing of proteins, obtained after extensive washes with buffers containing physiological amounts of salts, ruling out weak electrostatic associations (Fig. 11A).
These tissues may well serve as dynamic reservoirs of small physiological amounts of intravascular fluid.
Physiological amounts of purified as well as 435S cell-secreted NDPK also promoted angiogenesis that was attenuated by NDPK depletion or 10 μ M MRS2179, indicating a P2Y1 receptor-mediated pathway.
We could also show that a physiological amount of LPL, produced by macrophages, was capable of liberating MUFAs at a concentration high enough to cause apoptosis in endothelial cells [6].
"It can kill things directly, by physiological effects, of which ocean acidification is the best known, and it can kill things by changing the climate.
Pulmonary ventilation differs from alveolar ventilation only by the amount of physiological dead space, and there is no reason to suspect a change in physiological dead space when a dietary manipulation is applied.
Electroneutrality and physiological ionic strength of ∼0.150 M for the CaB simulations and 0.1 M for the free Ca2+ simulations were reached by adding appropriate amounts of Na+ and Cl− ions to the simulation boxes (as detailed in Table 3).
Heat is produced in large amounts by physiological oxidative reactions, and the blood is essential for its distributing and disposing of this heat.
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CEO of Professional Science Editing for Scientists @ prosciediting.com