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The resulting DNA fragment was subjected to nucleotide sequencing analysis and followed by phylogenic analysis using CLUSTAL X 2.0 (Larkin et al. 2007) and MEGA 6.0 software (Tamura et al. 2013).
The genotypes were determined by phylogenic analysis using the NS5B and E1 sequences.
To accommodate this, ca. 3000 mouse:rat pairs having f2Y and f2R values distributed around 0.88 and 0.90 (right mode of distributions in f2Y and f2R, Fig. 3) were used as mouse:rat orthologous pairs; this was confirmed by phylogenic analysis using the Homologene database (built in May, 2004) and the MasterCatalog (built in March , 2004.
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Phylogenic analysis using full length protein sequences showed that TCL2 is closely related to TCL1.
The resulting file was subjected to phylogenic analysis using the MEGA4.0 program [ 70].
Phylogenic analysis using the trimmed PX domain sequences does not reveal obvious sub-families.
To determine the relatedness of our elements with other DIRS1-like retrotransposons, we performed a new phylogenic analysis using the Maximum Likelihood method.
Phylogenic analysis using partial β-tubulin and calmodulin sequencing confirmed two of these as A. minisclerotigenes, while the third could not be differentiated from A. flavus and related species within Aspergillus section flavi.
The presence of HAV was further confirmed as subgenotype IB by phylogenic analysis of the sequence of the HAV-specific RT-PCR product.
Supported by phylogenic analysis, we distinguished novel families and identified a canonic representative element for each family.
This pattern of segregation was confirmed by phylogenic analysis, which showed that viruses detected in the United Kingdom formed a separate cluster with respect to the samples from Italy, Hungary, and Romania.
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