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Endotoxin was removed from the protein solutions by phase separation using Triton X-114 (Sigma-Aldrich) [49], [50].
Endotoxin was removed from the protein solutions by phase separation using Triton X-114 (Sigma-Aldrich, St . Louis MO) [49], [50].
The homogenate was incubated for 10 min at room temperature, followed by phase separation using 200 µL of chloroform and precipitation of the total RNA from the aqueous phase using 500 µL of isopropyl alcohol.
Endotoxin was removed from the protein solutions by phase separation using Triton-X 114 (Wako Pure Chemical Industries Ltd., Osaka, Japan).
Samples were resuspended in Tri-Reagent and washed with glass beads at temperature below 0°C followed by phase separation using 1-bromo-3-chloropropane.
200 μL Chloroform (Sigma Aldrich Chemie b.v., Zwijndrecht, The Netherlands) was added and the samples were incubated for 3 minutes, followed by phase separation using centrifugation at 21000 g for 15 minutes.
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The amino acid analysis was accomplished by precolumn OPA derivatization of the sample followed by reverse phase separation, using an Agilent 1100 HPLC (Agilent Technologies, Palo Alto, CA, USA) equipped with a thermo-controlled auto-sampler, fluorescence detector and an Agilent HP Chemstation for data elaboration.
PEUU and PEEUU were further fabricated into scaffolds by thermally induced phase separation using dimethyl sulfoxide (DMSO) as a solvent.
Porous polyurethane (PU) membrane was fabricated by the method of phase separation using water droplets produced from ultrasonic atomizer as coagulation bath.
The results show the control of the structures by appropriate synthesis and the monitoring of phase separation using dielectric spectroscopy.
The ZPs were prepared by phase separation process and optimized using uniform experimental design.
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