Sentence examples for by phase contrast using from inspiring English sources

These cultures were maintained with weekly two-third-medium changes, and after four weeks cobble stone areas were analyzed by phase contrast using a Leica DM IL HC microscope (Leica, Wetzlar, Germany).

For P-EYFP-N1 and P-EYFP-N1-SPG20 transfexperimentsriments, 10 15 different fields of cells were imaged by fluorescence and phase contrast using a 20× or 60× objective every 4 min for 8 hr.

Cell movement was followed by phase contrast microscopy using a 10× objective.

At various times samples were analysed by phase contrast LM using a CCD camera, by VCM or SEM.

The diversity of protozoa in 10-fold concentrated wastewater samples was also determined by phase contrast microscopy using a Leica DMR microscope after staining the samples for 1 h with 1 µL each of neutral red (100 µg/mL; Sigma-Aldrich) and acridine orange (1 µg/mL; Invitrogen), and diluting samples with an equal portion of glycerol to decrease protozoan motility.

Images were acquired by phase contrast imaging using a 10× N-Achroplan Phase contrast objective (NA 0.25).

Plates were incubated for 10 days and examined for granuloma formation by phase contrast microcopy using an inverted microscope.

In order to test whether or not reduction of Indy/CeNAC2 in C.elegans also shares characteristics of caloric restriction, whole body fat stores were analyzed by phase contrast microscopy using the azo compound Oil Red O to stain triglycerides.

After 24 hours, the cells were vigorously washed five times with phosphate-buffered saline (PBS), and the remaining cells attached to the plate were analyzed by phase contrast images using a Leica DMIRB microscope with a Hamamatsu C4742-95 Orcameraera.

Cell morphologies of treated and untreated cells were analyzed by phase contrast light microscopy using an inverted Axiovert 200 microscope.

Binucleate (BN) cells were quantified by phase contrast and fluorescent microscopy using an inverted Leica DM IRB microscope; CellMask (plasma membrane dye/Invitrogen) and AlexaFluor 488 Phalloidin were used to demarcate the cytoplasm.