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Each vessel was then recannulated and perfused with BSK-II medium followed by perfusion with B31-A3 spent medium.
Afterwards, they were transcardially perfused with phosphate buffered saline followed by perfusion with 4% paraformaldehyde.
Decellularization was achieved by perfusion of detergents and ionic solutions.
Furthermore, whole organ matrices can be isolated by perfusion decellularization.
Free aldehydes were then removed by perfusion with 100 ml of 10% sucrose in 0.1 M PB.
In an in vivo study, increased tissue regeneration was observed in TESTs fabricated by perfusion culture.
Recently, use of whole organ scaffolds obtained by perfusion decellularization has begun to draw interest.
The hearts were excised and mechanically arrested ex vivo by perfusion with BDM on a Langendorff apparatus at 12 weeks post-transplantation.
The CD34low subpopulations were not significantly altered by perfusion of the 3D scaffolds, neither on day 5 nor on day 9 (Fig. 3C).
Panigel, M., Pascaud, M. & Brun, J. L. adioangiographic study of circulation in the villi and intervillous space of isolated human placental cotyledon kept viable by perfusion.
Seven weeks after operation, hemodynamic measurements were made in the survivors (6 in each group) and the heart was fixed by perfusion with glutaraldehyde.
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