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These results were confirmed by performing the reverse experiment where IRS-1 was first immunoprecipitated, followed by western blotting with the anti-nitrotyrosine antibody.
Similarly, by performing the reverse experiment, immunoprecipitation of RSK1, YB-1 was detected.
JT took part by performing the reverse transcription reactions, purified PRV RNA, and propagated PK-15 cells.
By performing the reverse transcription step in digitized volumes, we find that the assay exhibits a linear signal across a wide range of total RNA concentrations and agrees well with standard curve qPCR.
We then confirm that the candidate pair (r E, ν E ) is in fact a strategy of equal invasion probability by performing the reverse experiment; that is, introducing a mutant cell with strategy (2.0, 2.5) into a background population of strategy (r E, ν E ).
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In all cases, the presence of gDNA was excluded by performing the adequate control reactions without reverse transcriptase.
The possible contamination of RNA with DNA was distinguished by performing the RT reaction without MMLV reverse transcriptase enzyme (-MMLV RT).
Genomic DNA false positive results were excluded by performing the reaction in the absence of reverse transcriptase, and with a treatment of RNA samples, prior to the RT-PCR reaction, with DNase I, RNase free.
We then performed the reverse experiment by switching flies back to non-RU486 containing food to stop induction of DN-Dmp53.
In addition, we performed the reverse experiment by overexpressing IL13Rα2 in the non-tumorigenic MCF10A (MI) cell line which resulted in inhibition of STAT6 activation by IL-13 (Additional file 6: Figure S5a, S5b).
In the early stages of the match, Virgil caused a distraction, allowing DiBiase to take the advantage by performing a reverse neckbreaker and throwing R-Truth into the barricade at ringside.
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