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We analyzed prominent fungal signaling pathways in Bgh, including: heterotrimeric G proteins/GPCR, MAPKs, cAMP signaling, small monomeric GTPases and Ca2+ signaling, by performing sequence similarity searches.
The structures were classified by structural similarity (defined as the number of aligned residues) and sequence similarity by performing sequence similarity searched against the Uniprot and Pfam databases.
europaeus and O. cuniculus) and by performing sequence similarity in National Center for Biotechnology Information using BLASTX (Altschul et al. 1997) against the nonredundant protein sequences database.
Thus, comparative positions of anonymous markers without direct human alignments were established by performing sequence similarity search on the human genome sequence with bovine WGS contig sequences containing the targeted marker sequences.
Novel paralogous domains were detected by performing sequence similarity searching with BLAST [ 54] among protein sequence regions found to be negative for current TIGRFAMs and Pfam HMMs, followed by single linkage clustering.
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By using this database, researchers can perform sequence similarity search (BLAST) with putative cryptochrome sequences to evaluate and determine conserved amino acids by the alignment of specific domains or full-length sequences using CLUSTALW.
We also performed sequence similarity searches using BLASTN against expressed sequence tags (EST others) [98].
Functional annotation of the cluster consensi was performed by sequence similarity searches using BLASTX program [ 36] against NCBI's non-redundant sequence database.
Manual annotation and analysis of the assigned function was performed by sequence similarity search using BLAST against the NCBI nr database, and assisted by conserved domain search (CD-search), identification of signature sequence motifs and sequence analysis using InterProScan.
Manual confirmation of the assigned function was performed by sequence similarity search using BLAST against the NCBI nr database, and assisted by conserved domain search (CD-search), identification of signature sequence motifs and sequence analysis using InterProScan.
To determine how related Cg3035 is to other known NAGS genes, comparisons between "classical" ArgA proteins, bifunctional ArgJ, S-NAGS and ArgO from Campylobacter jejuni[ 32] were performed by sequence similarity-based searches with the Cg3035 protein sequence using the basic local alignment search tool (BLAST) [ 33] at the NCBI.
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