More interestingly, they indicate that the prediction of linear motif instances can be enriched with contextual information by performing a sequence analysis similar to the one presented here.
The genetic variability of virus isolates was further investigated by performing nucleotide sequence analysis of the VP1 capsid region of isolates that were identified as possible VDPVs by the VDPV screening assay.
Phylogenetic relationships of D. zeae EC1 with other Dickeya species were assessed by performing Multilocus Sequence Analysis (MLSA) using four housekeeping genes (atpD, dnaX, gyrB and recA), and the concatenated data set of the four genes was constructed with Pectobacterium atrosepticum SCR1043 as an outgroup.
We examined the methylation states around the promoter regions of five pluripotency-related genes (OCT4, NANOG, SOX2, C-MYC and KLF4) by performing bisulfite sequencing analysis.
If rare nucleotide variations are indicated within a sample/family when analysing the parental DNA samples, this pitfall can be addressed by performing a sequencing analysis using alternative primer sets to generate the target PCR template.
Genotyping the TOE1 g.2171G>A SNP was performed by sequence analysis using the automated sequencer Genetic Analyzer 3500 (Genetic Analyzer 3500, Applied Biosystems by Life Technologies GmbH, Darmstadt, Germany).
Identification of yeast strains was performed by sequence analysis of the yeast internal transcribed spacer (ITS) regions.
Relevant data from these networks included transmission route(s) and norovirus genotyping performed by sequence analysis of the polymerase (ORF-1) and capsid (VP1/ORF-2) regiofs of the norovirus genome.
Unlike in previous reports (6, 7 ), characterization of hMPV strains in our study was performed by sequence analysis of a 633-bp fragment from the most variable hMPV G gene.
One reviewer suggested that performing a sequence analysis animal-by-animal in the goal-cue run session would be more conclusive than it is in the REST2 session.
