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By performing sequence alignment of TFEB homologues from different species, we found that K116 is highly conserved in evolution (Fig. 3D).
The SplitsTree decomposition network of pilE sequences was obtained by performing sequence alignment in MEGA 5.0 [ 74] and network analysis using Splitstree4 [ 75].
PTMBlast can be used to identify novel PTM sites by performing sequence alignment between user-defined PTM sites/peptides with different target datasets in SysPTM 2.0.
When a new sequence is found, the structure and function can be easily predicted by performing sequence alignment because a sequence sharing common ancestor would exhibit similar structure or function.
MetaGenome Rapid Annotation using Subsystem Technology (MG-RAST) is a freely available (http://metagenomics.nmpdr.org), fully automated system able to process metagenome sequence data by performing sequence alignment, sequence functional and phylogenetic assignments, and comparative metagenomics [ 13].
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The PABP1 RRM4 structure was generated by performing sequence alignment-based molecular superimposition on to the NMR solution structure of human PABP3 RRM4 (PDB code 2D9P).
There are currently several publicly available tools to detect evolutionarily conserved sequences across large genomic regions by performing sequence alignments [ 10, 11].
A possible application would be to take intron positions into account when performing sequence alignments by giving a higher score if intron positions are conserved.
ZJL performed sequence alignment.
Thus, comparative positions of anonymous markers without direct human alignments were established by performing sequence similarity search on the human genome sequence with bovine WGS contig sequences containing the targeted marker sequences.
Target similarity has been assessed at the level of protein sequence, with the degree of conservation of specific drug targets in humans and model organisms evaluated by performing sequence-by-sequence alignments, and limited studies have been conducted on the domain conservation for the androgen receptor (AR) and estrogen receptor α (ERα).
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