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We next addressed the use of a single tissue specimen per case as a potential methodological limitation by performing quadrant analyses on three parous cases >5 years postpartum.
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Apoptotic and necrotic cells were analyzed by performing quadrant statistics on PI-negative/annexin V-positive cells and PI/annexin V double-positive cells, respectively.
We further investigated the directionality of the ACC-FEF interactions by performing Granger-causality analyses.
Note that a finer differentiation between individual emotion categories within each quadrant has previously been established in larger population samples (Zentner et al. 2008) and was also tested in our fMRI study by performing additional contrasts analyses (see below).
Cellquest Pro was used to perform quadrant analysis.
The data files were saved automatically by CellQuest software (BD Biosciences), and WinMDI (version 2.8; The Scripps Institute, Flow Cytometry Core Facility, La Jolla, CA), was used to perform quadrant analysis.
Cell cycle distribution in histogram and Annexin V quadrant analyses were analyzed by using the WinMDI software (designed by Dr. Joseph Trotter, the Scripps Research Institute, USA, version 2.8).
They talk about performing cohort analyses.
Significance analyses of two groups were performed by Mann Whitney analyses.
Based on these interactions, we performed subgroup analyses by BRCA2 status.
W.Z. performed electrophysiological analyses.
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