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The annotation information was enriched by performing multisequence alignment using CLUSTALW [ 19].
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Differentially expressed rice genes were functionally annotated by performing BLAST alignment against the TIGR database.
The spacer sequences were compiled and aligned by using the multisequence alignment program ClustalX (1.8).
Sequencing products were purified and underwent electrophoresis with the 3100 Genetic Analyzer (Applied Biosystems) and aligned by using the multisequence alignment CLUSTALX version 1.8 (13 ).
The multiple alignment of amino acid sequences was performed by ClustalW.
Sequence alignments were performed using the ClustalW multisequence alignment program, available on-line from the EMBL-EBI (http://www.ebi.ac.uk/clustalw/) [34].
The reproducibility of the alignment approach was tested by performing the mapping step with BWA, an alternative alignment program [ 45].
In order to identify residues in the cap region which are conserved between the "Candida antarctica lipase A like" and "Deacetylases" superfamilies, a multisequence alignment of each family was performed.
Each of the putative RCRRs were evaluated for the quality of the local multisequence alignment by PLOTCON from the EMBOSS suite [ 28] with the EBLOSUM62 matrix.
Sequences were aligned using the MUSCLE multisequence alignment program.
They could be identified and annotated by a manual validation of each family-specific multisequence alignment.
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