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In addition to the high-affinity 10-mer motif, we found a 10-mer sequence, CAT-GACTAAT, by performing multiple sequence alignment of 4 other 7-mers identified through Round 1 (i.e., ATGAGTC, TGACTAA, GACTCAC, and GACTAAT).
Sequence conservation of the dockerin was demonstrated by performing multiple sequence alignment of the 75 type I dockerins, and creating a sequence logo of the two repeats of the dockerin modules.
We performed secondary structure analysis with Coils (Lupas et al., 1991) and PSIPRED (Buchan et al., 2013), and we identified regions of sequence conservation by performing multiple sequence alignments with Clustal W2 (Larkin et al., 2007); appropriate deletions were designed that removed conserved structural regions.
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We performed multiple sequence alignments by using the ClustalX program (www.clustal.org/clustal2/).org/clustal2/
The MUSCLE (Multiple Sequence Comparison by Log-Expectation) program (version 3.52) was also used to perform multiple sequence alignments to confirm the ClustalX data output [ 106].
We performed multiple sequence alignments to investigate the conservation of the domain regions by ClustalW [ 51].
A second program, mpi_seq_router.pl, performs multiple sequence processing in parallel.
The conservative motifs among AP2/ERF proteins in plants were clarified by performing multiple alignment analyses using amino acid sequences of the AP2/ERF domain.
Phylogenetic analysis was carried out by performing multiple alignments of full-length DHN protein sequences from V. vinifera V. yeshanensis H. vulgare and Arabidopsis using MEGA5 [ 68].
To confirm the PCR results, we sequenced the amplicon in both directions in 2 independent setups and compared the obtained 299-bp sequence with published sequences from GenBank by performing a multiple sequence alignment.
We conducted sequence-based analyses to identify these amino acid residues by performing a multiple sequence alignment and using the AVANA tool (http://sourceforge.net/projects/avana/) to compare the mutual information between subsets of the alignment for the location of the characteristic sites [17].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com