To better understand the mechanistic role of miR-22 in gastric carcinogenesis, we performed miR-22 overexpression experiments by miR-22 transfection in the SGC-7901 celineine, in which miR-22 expression was lowest among four GC cell lines.
A broad miR expression array (379 miRs + U6) was performed for global miR profiling using one randomly selected patient (# 101) that required 500 µL plasma at baseline and post dovitinib treatment (Qiagen Valencia, CA).
MiR expression was quantified by qRT-PCR, using the SYBR green PCR Master mixture (Qiagen cat.
Neerincx, M. et al. MiR expression profiles of paired primary colorectal cancer and metastases by next-generation sequencing.
Therefore, multivariate survival analysis was performed by using miR-34a expression and pN status.
To gain further insight into which genes were affected by miR-874 transfection, we performed gene expression analysis with miR-874 transfectants and the controls in IMC-3 cells.
To gain further insight into which genes are affected by miR-1 transfection, we performed gene expression analysis with miR-1 transfectants and the controls (BOY and T24 cells).
The over-expression experiments were performed with miR-1, miR-30a, miR-155, miR-16, let-7b, miR-124, miR-181, among which the miR-1 over-expression were carried out by both studies [6], [7].
BLT performed the miR-24 expression analysis and anti-miR-24 siRNA experiment, and KHT performed all other experiments.
Besides miRs expression analysis, we also performed a comprehensive study of the histology and immunohistochemistry related to the epicardium and CM differentiation.
