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In order to begin to investigate the mechanisms underlying the promotion of migration by PBMC in adult OA cartilage, we performed gene array analysis using a human cell motility PCR array.
Evaluation of the Notch and Wnt pathways in CRC explant tumours was performed by gene array and immunoblotting.
The corresponding quality-tested total RNA was used by the Lausanne DNA Array Facility (DAFL) to perform gene expression profile analysis on Affymetrix HG-U133 Plus 2.0 Arrays, according to the manufacturer's recommendations (http://www.unil.ch/dafl/).unil.ch/dafl/
TA provided resources and performed gene expression analysis by Affimetrix gene array.
MD and PA performed the gene array analysis.
The arrays were normalized as per typical two-color experiments by performing an array-wide intensity-dependent Lowess normalization, followed by a per gene normalization, which normalized each spot to the median value.
In an attempt to further identify the possible PAK4 regulation of different essential molecular intermediates participating in tumor cell proliferation, adhesion and anoikis resistance, we analyzed the gene expression levels by performing cDNA-PCR arrays in pSV- and PAK4si-treated 4910 cells.
Regulation of apoptotic genes was determined by performing PCR-based arrays and RT PCR.
To determine the distinct pattern of gene expression after activation of NFκB signaling by vinclozolin treatment, we performed pathway-specific gene array analysis of 113 key genes involved in the inflammatory response.
We studied the performance of the Gene Array and the Exon Array by comparing previously published experiments performed on the MAQC RNA samples (see Methods).
XL performed analysis of gene array.
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