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Some of these predicted cellulases have been previously verified experimentally [22], [26] [30] and we contributed to this growing list by performing cloning, expression, and carbohydrate hydrolysis assays on other putative glycoside hydrolases as shown in Table 2 and S2.
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Eva Ullmann and Thomas Gundinger performed cloning, expression and biochemical experiments.
SR and ND performed cloning, expression analyses and database searches.
MD performed the cloning, expression, purification and characterization experiments.
ME, AD and DC performed cloning, fly crosses and analysis, and antigen expression.
NW performed cloning.
Always perform cloning tasks first.
Cloning efficiency was improved by performing an intermediary cloning to ensure highest incorporation of full antibody gene sequences into the destination phagemid vector.
ST-3 cDNA was amplified by performing a PCR and cloning into the mammalian expression vector pcDNA3.1V5HisTOPO (Invitrogen, Karlsruhe, Germany).
VK performed the experiments regarding cloning, expression, purification and assay of Ed EN.
The expression clone was constructed by performing a Gateway LR reaction with the above two entry clones, pCR347 and pCR319.
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