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Optimal kmer length was determined by performing assemblies on one genotype at a time and each of the three Illumina read sets was assembled separately.
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We performed assemblies on these datasets using Edena [ 12], Velvet [ 4], Contrail [ 10] and CloudBrush assemblers.
UB performed assemblies on the compute cloud and assisted in drafting the manuscript.
In total, we performed assembly tests on the following three sample sets.
In this study, we investigated the factors that govern MCP assembly by performing scanning mutagenesis on the surface residues of PduA, a major shell protein of the MCP used for 1,2-propanediol degradation.
The U-type assembly line is an attractive substitute for assembly production systems from the time operators became multi-skilled by performing tasks defined on different parts of assembly line (Gökçen et al. 2005).
JFP performed assembly by Ray.
Pell et al. did not perform assembly directly by traversing the probabilistic graph.
The combined FW&SW gill transcriptome was generated by performing de novo assembly using CLC Bio Genomics Workbench (version 5.1) on the combined FW&SW library [see Methods].
Unique sequences were grouped into operational taxonomic units (OTUs) by performing a contig assembly in Sequencher v.4.1.4 (GeneCodes, Ann Arber, Michigan, USA) based on a 97% similarity criterion.
For each simulation, the compositional space was sampled by performing 600,000 SGPs based on 30,000 parent assemblies, as detailed: 10,000 parent assemblies were generated at random, each by randomly picking a molecular type and adding a random count of this type until the assembly size reaches Nmax.
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