Sentence examples for by performing a three from inspiring English sources
The Ca2+ dissociation constant of fura-2 in Merkel cells was determined by performing a three point calibration in situ with solutions containing (in mM): 135 KCl, 2 MgCl2, 10 HEPES, and one of the following: 10 EGTA (for Rmin), 10 CaCl2 (for Rmax), and 8.5 EGTA plus 1.5 CaCl2 (for Rmid, effective free [Ca2+] = 0.9 µM as measured in vitro by fura-2 imaging).
We analyzed the genomic regions encoding these orthologs by performing a three frame translation of the genomic sequences, and using the gene prediction program genescan [ 41] as well as a splice site prediction program [ 42].
Synergism in the time course was determined using the cumulative cytokine levels after 48 hours by performing a two way ANOVA and examining the interaction term.
By performing a two step hybridization, several clones were retrieved and sequenced.
Potentially relevant articles were obtained by performing a search in three databases (PubMed, CINAHL, and EMBASE) from January 1975 until April 2010.
Patients were videoed in a standardized manner by performing a set of ten active movement tests.
We tried to verify these results by performing a cost analysis in three European countries.
Robustness of this cluster was demonstrated by performing a TCA with these five genes (Table 4).
Where appropriate, data were evaluated by performing a simple comparison between two values using Student's t-test.
This was achieved by performing a full-scale testing of five floor specimens.
