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This list of QTLs shared by the camptothecins was further refined by performing a third validation study with seven of the nine drugs from our initial study (Camptothecin Group A/B: mCPT, hCPT, 9AC, 9NC, SN38, CPT, TPT).
As ultra-deep sequencing of NA12760 and NA18502 amplicon F5 provided most consistent CN estimations (Table 2, Additional file 1, Table S7) for samples with high CNs, we further validated this approach by performing a third GS FLX run with pooled amplicons F2, F4, F6 and F8 from these DNAs.
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The validity of measurements has been demonstrated by performing a first series of tests and numerical simulations.
In experiment 3, we tested the cross-cultural consistency of our findings by performing a second replication with Brazilian participants.
However, our experiments indicate that the improvement gained by performing a second iteration is not significant enough to justify the additional complexity.
After by-passing the regression to the mean artifact, by performing a second glutathione measurement, the individuals were supplemented with NAC (2 × 600 mg, twice daily, for 30 days) or placebo using a double-blind cross-over design.
The new scheme is derived by performing a fourth-order finite difference (FD) approximation to the spatial derivatives of the axisymmetric fluid diffusion equation in a non-uniform grid configuration.
This can either be done manually, by use of pair-wise distance analysis, or by performing a second parsimony analysis using data of a single genus only.
We confirmed all positive results by performing a second qPCR targeting the IS 30a repeated sequence.
Pre-formed RNA DNA hybrids were made by performing a first strand synthesis reaction on total RNA.
The obtained contigs and singletons were further re-assembled by performing a second round to decrease the redundancy caused by the heuristic nature of the assembly process [ 34].
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