Abbreviations: FISH, Fluorescent in situ hybridization; HPV, Human Papillomavirus; PBMC, Peripheral Blood Mononuclear Cells; SB: In order to investigate the molecular phenotype of HPV bearing cells, we enriched samples in round cells by performing a density gradient centrifugation of whole semen.
The optimal annealing temperature for the PCR reactions was determined by performing a temperature-gradient-PCR in a range from 50-68°C 50-68°C
In addition, SST gradients were calculated by performing a two-dimensional convolution on the GOM bathymetry grid with a 3×3 Sobel filter [23], [24].
This method overcomes the difficulties arising in the project gradient method by performing an additional forward step and a projection at each iteration according to double projection.
A local minimum of the objective function can be found by performing gradient descent in W and L. Afterwards, more familiarity scores between workers and landmarks are inferred in M. A worker with a familiarity score of a landmark means that he has some knowledge about the region around the landmark, not just the landmark itself.
By performing gradient elution with unbuffered acids, ERLIC was optimized for whole proteome fractionation [30].
A final extension was for 5 min. Optimal annealing temperatures were determined by performing gradient PCR on each of the primer sets.
The CNS package (37) was used for the refinement of the models by performing simulated annealing, gradient minimization, refinement of individual isotropic temperature factors, and individual occupancy.
Using these two technologies, and by performing analyses of gradient fractions in the presence or absence of proteinase K, we compiled an inventory of oligomeric PrPSc species: this inventory included net levels of protease-sensitive and protease-resistant (r) forms of PrPSc (rPrPSc), as well as PrPC levels in fractions at the top of the gradients (Tables 1 and 3).
Optimal hybridization temperatures were determined by performing reactions over a 10°C gradient.
