Sentence examples for by performing a dilution from inspiring English sources

The optimal concentrations of siRNA and time points were determined by performing a dilution curve of siRNA for each sequence and the knockdown were determined by Western blot analysis.

The efficiencies of target gene and GAPDH amplification were investigated by performing a dilution series of total RNA (1 μg to 0.1 ng) following recommendations [ 84].

Finally, we measured the sensitivity of our mutation detection assay by performing a dilution of mutation template into increasing amounts of wild-type genomic DNA lacking the mutation.

PCR efficiency (E) was evaluated by performing a dilution series experiment using a target assay and the Equation E = 10(−1/slope)[ 65].

PCR efficiency (E) was evaluated by performing a dilution series experiment using a target assay and the equation E = 10(−1/sloPfafflfaffl 2001).

Great care was taken to rule out other contributions to the SAXS signal such as a structure peak from particle aggregation or dumbbells present from the synthesis by performing a dilution series and studying the temperature response of the size of the shell.

To functionally prove that CSCs are killed by dimerization of iCasp9, we analysed the clonogenic capacity of CSCs after dimerization treatment by performing a limiting dilution assay (LDA).

CFUs in the BAL were estimated by performing a serial dilution of BAL fluid in 0.1% Tween-20/dH2O and plating onto Sabouraud agar (Oxoid, UK) plates.

qPCR primers were resuspended at 500 nM each and validated by performing a tenfold dilution curve qPCR, starting with 2000 ng cDNA, down to 2 ng cDNA, in duplicate.

To investigate the effect of diluting over-range samples into the calibration range, the accuracy and precision of dilution samples at 20,000 ng/mL (plasma samples), 10,000 ng/mL (small intestine homogenate) and 5000 ng/mL (liver homogenate) (each n=5) were assessed by performing a 20-fold dilution.

Before performing the data normalization, we checked the PCR efficiencies of all miRNA assays on the array cards by performing a 10-fold dilution series and subtracting the Ct values of the undiluted sample from the Ct values of the diluted sample.