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The residual DNA was eliminated by performing a column DNase digestion at 37°C for 30 minutes.
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In consequence, retention time stability was further increased by performing a 20 min column regeneration step after each run before the column was equilibrated to starting conditions for 90 min.
Ottawa, Ontario, Canada) and proteins were purified by performing an affinity column chromatography with subsequent cleavage of the GST tag by using PreScission Protease (GE Healthcare, Waukesha, WI).
Residual DNA was removed by performing an on-column digestion using an RNase Free DNase (QIAGEN GmbH, Hilden, Germany).
By performing a convenient change on the columns of H and G matrices, all known variables are placed at the right hand side of this algebraic system, whereas unknown variables, x, are placed at its left hand side (Leonel and Venturini 2011).
We first match inferred mixture components to true mixture components by performing a maximum weighted bipartite matching of columns between Ctrue and the inferred components C e, weighted by negative Euclidean distance.
We collected the topics in the trends column by performing an API query every 20 minutes.
By performing an original?
Residual genomic DNA was removed by performing on-column DNase I digestion by using the RNase-Free DNase set (Qiagen).
Finally, sc MC was isolated from oc DNA molecules (oc MPs, oc MC) and RNA by performing HIC with a phenyl-Sepharose column using a series of elution steps with decreasing ammonium sulfate concentrations.
From Equation 2, it can be observed that L can be calculated by performing PCA on the columns of matrix U.
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