Sentence examples for by performing a cell from inspiring English sources
Furthermore, by performing a cell surface biotinylation assay we ensured that at least a detectable fraction of all the TRKB mutants properly localized to the cell membrane [36] (Figure 1D).
This cell number was empirically determined for our cells by performing a cell titration assay.
We also were able to demonstrate the sensitivity of the platform by performing a cell mixing experiment.
The cytotoxicity of each lipid electrophile was determined by performing a cell viability assay and generating a concentration−response curve.
The TBP reference primers have also been previously validated (Nygard et al., 2007) Cells were characterized by performing a cell proliferation assay, immunocytochemistry (Rasmussen et al., 2011) and comparative qPCR.
Specifically, we transfected a library of 316 microRNAs and two microRNA negative controls (miR-NC) and 48 h post transfection, SNU-449 cell invasiveness was measured by performing a cell invasion Boyden chamber assay (Fig. 1b).
To understand whether inactivation of BRCA1 by germline mutations affects the TGF-β signaling pathway, we investigated the effect of BRCA1 mutations on TGF-β1 transactivation activity by performing a cell-based reporter assay with a TGF-β-responsive reporter, p3TP-Lux [19].
The suppressive function of Foxp3+CD4+ Tregs was determined in vitro by performing a T-cell proliferation assay.
In order to increase our understanding of the regulation of p53 we searched for novel, evolutionary conserved regulators of p53 and Mdm2 abundance by performing a mammalian cell-based overexpression screen using a cDNA library obtained from the teleost Medaka.
The IGL cell density was quantified in ImageJ by performing an automatic cell count of propidium-iodide-stained sections with specified parameters for particle size and labelling intensity, and expressed as number of cells per μm.
The decrease in β1-integrin RNA was confirmed at the protein level by performing a fluorescence-activated cell sorting analysis on intact cells with an antibody that recognizes active β1-integrin (see Additional data file 9).
