Prior to sorting, aggregates were removed by passing through a 40 µm cell strainer.
Urate was dissolved in 0.4 N NaOH and sterilized by passing through a 0.2 μM nylon syringe filter.
Single-cell suspensions were generated by passing through a 40 μm filter mesh, and the resulting suspension washed and incubated with 0.86% NH4Cl in PBS to lyse erythrocytes.
The supernatants were collected and condensed from 50 mL down to 1 mL using a Büchi Rotavapor-R apparatus, then sterilized by passing through a 0.2 micron filter.
It was humidified by passing through a separate liquid water container before entering the cell.
At this point the carcasses are usually singed by passing through a flame that burns off any remaining feathers.
Lysates were homogenized by passing through a 28G syringe, mixed with 6X sample buffer, and stored at −20 °C.
The nematodes were collected from the water, then concentrated by passing through a 20-μm sieve.
By our assumptions, we can assume, by passing through a subsequence, that for some.
and we can assume, by passing through a subsequence, that for some.
Then, the filtered signal is digitized by passing through a bandpass sampling ADC [3].
