Sentence examples for by passaging through a from inspiring English sources
Cell clumps were removed by passaging through a 50 µm nylon filter.
The tumor cells were isolated from excised skin tumors by passaging through a cell sieve to remove connective tissue and MACS with antibodies for the Vβ family of the tumor cells as previously established by immunohistochemistry.
Cells were then dissociated by passaging through a fire-polished pipet and plated on collagen-coated dishes in MEM with Earle's salts supplemented with 50 ng/mL NGF, 10 % FBS, 2 mM glutamine, 100 µg/mL penicillin, 100 µg/mL streptomycin, 20 µM flourodeoxyuridine, 20 µM uridine, and 3.3 µg/ml aphidicolin.
The cells were lysed by passaging through a 27.5 G needle several times.
Single cells were collected by passaging through a 40-μm filter and centrifugation at 280 × g for 4 minutes.
The pellet was re-suspended with 1 ml of the base buffer solution supplemented with CaCl2, MgCl2, and protease inhibitor and lysed again by passaging through a ¾ inch 23 gauge needle, 20 times.
33 Genomic DNA was further purified by passaging twice through a 20/G genomic-tip according to the manufacturers instructions (Qiagen).
Concentrated SAPs were then purified by passage through a Pierce Strong Anion Exchange Spin Column (#90011, Thermo Fisher Scientific, Waltham, MA).
A yield of fine fiber from the retted stems processed through the Flax-PP was acquired from further cleaning and refining by passage through a Shirley Analyzer.
Since the 1950s levels have been introduced in which the line of sight is automatically leveled by passage through a system of prisms in a pendulum, thus removing the need to check the bubble.
