Sentence examples for by passage two from inspiring English sources

In contrast, by passage two in 400 µM ribavirin, the G64S mutation is present in 7% of viral genomes.

Moreover, with prolonged culture of Mbd3 knockdown cells three days after shRNAs transfection in TS cell medium, we observed formation of flattened TS-like colonies by passage two (Figure 6a, f) which expressed trophectoderm cell surface marker Cadherin3 (Cdh3, also known as placenta Cadherin) (Figure 6d, i).

Cells in 50 mM Hepes buffer, pH 7.5, were broken by passage two times through a French pressure cell at 15 k psi.

By contrast, exogenous gene expression was turned off in all iPSC lines by passage six (Fig. 2B).

pAFP1200 was transferred into C. difficile M68 by conjugation (4, 7, 21), and single-crossover clones were obtained by passage three times on selective media as described previously (3, 4) (Fig. 1B).

By the fifth nonselective subculture, 1 clone of the 50 replica-plated clones was Tms Lms and one clone Tms Lmr, indicating that one mutant and one wild-type revertant had been isolated; by passage six, 8 of the 75 replica-plated clones were Tms Lms and two were Tms Lmr.

Already disheartened and confused by the passage five weeks ago of a statewide referendum to dismantle bilingual education, Arizona's bilingual educators were further battered by a letter that appeared recently in The Arizona Daily Star.

For this purpose, a new system was developed to produce high titers of the HSV-1 amplicon vector and the results showed that its package efficiency and the titer ratio to HF10 were improved by passage through two cell lines.

After 60 min of incubation at 37 °C, DNA was purified by passage through two Bio-Gel P-30 columns.

Whole human serum was depleted of endogenous MBL and ficolins before setting up the assay by passage through two GlcNAc-Sepharose columns (0.5 for 1 mL of serum).

[F]fluoride was easily removed by passage through two disposable alumina cartridges, eluting with water or saline, as demonstrated using the TLC (Fig.  1) and HPLC (Fig.  2) methods described above.