Sentence examples for by passage through two from inspiring English sources
For this purpose, a new system was developed to produce high titers of the HSV-1 amplicon vector and the results showed that its package efficiency and the titer ratio to HF10 were improved by passage through two cell lines.
After 60 min of incubation at 37 °C, DNA was purified by passage through two Bio-Gel P-30 columns.
Whole human serum was depleted of endogenous MBL and ficolins before setting up the assay by passage through two GlcNAc-Sepharose columns (0.5 for 1 mL of serum).
[F]fluoride was easily removed by passage through two disposable alumina cartridges, eluting with water or saline, as demonstrated using the TLC (Fig. 1) and HPLC (Fig. 2) methods described above.
Microtiter plates (Nunc) were coated with 2 μg mannan overnight and incubated with 1/100 dilution of whole human serum, previously depleted of endogenous lectins by passage through two mannose-Sepharose columns (2 × 0.5 ml matrix/mL of serum) in 4 mM barbital, 145 mM NaCl, 2 mM CaCl2, 1 mM MgCl2, pH 7.4, supplemented with recombinant human MBL or human CL-K1.
Four-micron sections were cut from each TMA, mounted on SuperFrost® Plus glass slides and baked for 2 h at 79°C, then dewaxed by passage through xylene (two 5 min washes), cleared and rehydrated in graded alcohol (100, 95 and 70%) ending in a distilled water wash.
The cells were lysed by passage through a French press three times at 8000 lb in−2.
Cells were lysed by passage through a 25G syringe in one cell volume of 2× binding buffer (40 mM HEPES pH 7.4, 0.2 mM sodium orthovanadate, 100 mM NaCl, 0.4 mM EDTA, 2 mM DTT, 20 mM PPi, 100 mM sodium fluoride, 100 mM β-glycerophosphate, 1 mM AEBSF and 20 µg/ml of aprotinin and leupeptin).
Four parasitized monolayers were harvested every 24 hours by trypsinization and parasites purified by passage through a 21 gauge needle and washing in phosphate-buffered saline (PBS).
After close contact between the two immiscible phases for proper mass transfer, the oil water segments leave the oven through the restrictor, then are cooled by passage through a coil located in a bath at room temperature.
