Sentence examples for by passage over from inspiring English sources
A second treatment with chlorine liberates bromine, which is freed from chlorine and purified by passage over moist iron filings.
RNA was precipitated with isopropanol and purified by passage over RNeasy columns (Qiagen).
Cre reactions were stopped by addition of SDS (0.1%), and samples were switched to the sequenase reaction buffer by passage over QIAquick PCR purification columns (QIAGEN).
DO11.10 and ICOS−/− DO11.10 mice were sacrificed, and spleen and lymph node cells were enriched for T cells by passage over a nylon wool column [54].
To investigate the role of IgG in mediating chemiluminescence activity, seven sera with high ADRB values were individually depleted of IgG by passage over Protein-G, and the flow-through was concentrated to the original volume (IgG depleted serum).
Fractions containing target protein were pooled, and the N-terminal hexahistidine tag was removed by incubation with His-tagged TEV protease (molar ratio 30∶1) over night at 4°C, followed by passage over a 1 ml HiTrap Chelating HP column pre-equilibrated with buffer 1 with 25 mM imidazole.
Tumour-infiltrating lymphocytes (TIL) were isolated and purified by successive passage over Sephadex G-10 columns and their cytotoxic activity in vitro compared with that of lymphoid cells from normal rats and from tumour-bearers at different times after implantation.
The soluble DR was purified by passage of the supernatant over an affinity column coupled with the anti-DR antibody LB 3.1.
Antibodies were purified by passage of the antisera over a Sepharose 4B column that had been coupled to the peptides according to the manufacture's protocol (Amersham Biosciences Europe GmbH, Freiburg, Germany).
Most damage associated with Dennis was from the heavy rainfall caused by its slow passage over Florida, although two tornadoes were also reported within Florida.
Cut proteins were further purified by gel filtration on a Superdex 200 16/60 column (Pharmacia) equilibrated with buffer A followed by a second passage over nickel sepharose.
