Sentence examples for by partial digestion of from inspiring English sources

The library was generated by partial digestion of the BAC with CvIJ and gaps were filled-in by cloning of PCR fragments.

As well, a BAC library was constructed by partial digestion of genomic DNA with EcoRI and cloning in pBACe3.6 [ 81].

This gene library was constructed by partial digestion of genomic DNA from R. erythropolis NCIMB 11540 by Sau3A1.

Again, the 0.9-kbp EcoRI– HindIII fragment produced by partial digestion of this plasmid was cloned into pRS315 to produce pRS315– rfa2 -A x-Δpromoter.

A BAC library of P. virgatum was constructed by partial digestion of nuclear DNA with EcoRI and contains 147,456 clones stored in 384 plates (384-well).

The library was created by partial digestion of high molecular weight genomic DNA with HindIII according to the procedure of Peterson et al. [ 33].

The released DNAs and DAMPs was significantly increased by partial digestion with micrococcal nuclease (MNase), indicative of connection of released nucleosomes and DAMPs to cellular bodies.

They were then digested with the endonucleases, NcoI and HindIII, by partial digestion [71] due to the presence of these two sites in both cDNAs.

Two of the libraries (HVVMRXALLhB and HVVMRXALLhC) were derived by partial digestion with enzyme HindIII, whereas the remaining was derived from partial digest with EcoRI (HVVMRXALLeA) or MboI (HVVMRXALLmA) (Table 1), respectively.

Finally, one of the EcoRI sites was eliminated by partial digestion, end-filling with Klenow enzyme, and ligation to generate plasmid pHTH22 (Figure 1A and 1B).

Chromosomal DNA was isolated and prepared for cloning by partial digestion with several enzymes as listed in Table 2. Calculations of site frequencies and prediction of the number of 5' ends are based on published genome sequences.