Sentence examples for by partial denaturation of from inspiring English sources

This adsorption was attributed to hydrophobic interactions caused by partial denaturation of the protein, and it was greater for as-received ferritin containing higher oligomers than for monomeric ferritin.

To evaluate effects caused by partial denaturation of the antigen, biotinylated Crf2 was bound to 96 well streptavidin microtitre plates (Streptavidin F96 Clear, Nunc) in a parallel approach.

This phenomenon can be explained, in our opinion, by partial denaturation of serum proteins, leading to increased epitope accessibility for the antibody.

The digestion efficiency can be enhanced by partial denaturation by addition of 35 45% acetonitrile to the buffer [ 11, 15, 35, 36] or by sonication [ 42, 43].

After removal of RNA and partial denaturation of DNA by HCl, AO was used to stain nondenatured dsDNA and denatured ssDNA, resulting in green and red fluorescence, respectively.

This partial denaturation of proteins by immobilization [44] is only rarely described.

Quantification of antigen by ELISA, however, can be affected by the presence of IC, as well as partial denaturation of antigen with dissociation buffer.

Selective inactivation of the active center in the C-domain and partial denaturation of this domain was confirmed by analysis of mutant Q1069R ACE inhibition by ACE inhibitor enalaprilat and by anti-N-domain catalytic mAbs 3A5 and i2H5 (Fig. 8).

The interpretation of our results on the molecular level shows the importance of partial denaturation of recPrP as substrate as described by Stöhr et al. [11].

Often, adsorption induces partial denaturation of the protein, although this effect is less pronounced on neutral hydrophilic surfaces [23].

It was discussed earlier that the buffer conditions allow a particular extent of partial denaturation of recPrP in a well characterized pre-amyloid state.