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Efficacy of DNA extraction and amplification was examined by parallel amplification of the human APOB gene.
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A standard curve for each primer set was generated by parallel amplifications of plasmid template dilutions, and the average copy number of triplicate PCR reactions for each sample calculated from this.
By contrast, q-RT-PCR requires a parallel amplification of a housekeeping gene (HKG) as control gene (CG) to correct variations in RNA quality and quantity and to calculate the sensitivity of each measurement.
To allow parallel amplification of complete DNA threads, generic amplification handles were appended to the 5'-ends of the extension primers and to the 3'-ends of the thread-joining primers.
Parallel amplification of two human reference genes, L13 and cyclophilin, confirmed negative results in clinical samples by demonstrating amplifiable RNA.
Parallel amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control.
The presence of these adaptors enables the amplification of random-sequence inserts by parallel PCR amplification of millions of individual DNA molecules.
Diagnostic PCRs to detect point mutations consisted of two parallel amplification reactions.
By utilizing a solid-state electrode, patterned with standard photolithography and evaporated onto the chip, we added the potential for massively parallel amplification detection.
by rapid amplification of cDNA ends (RACE).
Further confirmation was done by PCR amplification of ITS region.
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